Rutinis a flavonol,abundantly found in plants, such as Sophora flower bud, buckwheat, tea, and apple.It has demonstrated a number of pharmacological activities,including antioxidant, cyto-protective, vaso-protective, anti-carcinogenic,neuroprotective and cardio-protective activities. Besides, rutin is a safe and natural antioxidant and sun-screener.

Rutin penetration through the fibroblast phospholipid bilayer was aided by UVA-induced Bili-translocase activity 2-4h after irradiation, while UVB irradiation led to enhanced phospholipid peroxidation and higher membrane permeability to facilitate the interaction of rutin with phospholipids (PLs). Lipidomic analysis revealed that 4h of rutin treatment also partially prevented UVA/B-induced increase in phosphatidylethanolamine(PE) and phosphatidylcholine(PC) level, as well as their membrane localization, which resulted in an enhanced zeta potential in the cells and liposomes. Moreover, rutin 2h following irradiation, in a various degree, prevented the increased in phospholipase A2(PLA2) activity and ROS generation, and partially protected against the reduction of arachidonic and linoleic acids level and the lipid peroxidation product 4-hydroxynonenal(4-HNE) level increase. Rutin effectively prevented against decrease in glutathione peroxidase (GSH-Px), glutathione(GSH) and vitamins E (Vit. E) and C activities/levels, particularly 2h following UVA irradiation.

In conclusion, highest skin fibroblasts membrane level of rutin occurred in 2-4h following UVA/B-radiation results in its strongest effect on biomembrane structure and functions and cellular antioxidant system irrespective of the radiation type.